Scatter plot
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Analytical conditions
The plant extracts (1 ml) were analyzed using LC-QTOF/MS (LC, Waters Acquity UPLC system; MS, Waters Xevo G2 Q-Tof).
LC
Column
Acquity bridged ethyl hybrid (BEH) C18
1.7mm, 2.1mm, 100mm
Waters
Solvent system
Solvent A
water including 0.1%
formic acid
Solvent B
acetonitrile including 0.1%
formic acid
Gradient program
99.5%A/0.5%B at 0min
99.5%A/0.5%B at 0.1min
20%A/80%B at 10min
0.5%A/99.5%B at 10.1min
0.5%A/99.5%B at 12.0min
99.5%A/0.5%B at 12.1min
99.5%A/0.5%B at 15.0min
Flow rate
0.3ml/min at 0min
0.3ml/min at 10min
0.4ml/min at 10.1min
0.4ml/min at 14.4min
0.3ml/min at 14.5min
Column temperature
40 ℃
MS detection
Polarity
positive/negative
Capillary voltage
positive
+3.00 kV
negative
-2.75 kV
Cone voltage
25.0 V
Source temperature
120 ℃
Desolvation temperature
450 ℃
Cone gas flow
50 l/h
Desolvation gas flow
800 l/h
Collision energy
6 V
Mass range
*m/z*
50–1500
Scan duration
0.1 sec
Inter-scan delay
0.014 sec
Data acquisition
Centroid mode
Lockspray
Compound
Leucine enkephalin
Scan duration
1.0 sec
Inter-scan delay
0.1 sec
MS data
Polarity
positive/negative
Mass range
*m/z*
50–1500
Scan duration
0.1 sec
Inter-scan delay
0.014 sec
Data acquisition
Centroid mode
MS/MS data
Polarity
positive/negative
Mass range
*m/z*
50–1500
Scan duration
0.02 sec
Inter-scan delay
0.014 sec
Data acquisition
Centroid mode
Note
In this mode, MS/MS spectra of the top 10 ions (> 1000 counts) in an MS scan were automatically obtained. If the ion intensity was less than 1000, MS/MS data acquisition was not performed and moved to of next top 10 ions.
The authentic standard compounds were analyzed by four different collision energy settings, 10 V, 30 V, 50 V, and ramped from 10 to 50 V.
The plant tissues were analyzed by one collision energy setting, ramped from 10 to 50 V.
